from PIL import Image import os img = Image.open('/home/enne2/dev/mice/assets/Rat/BMP_MALE_UP.png').convert('RGBA') pixels = img.load() w, h = img.size print(f"Size: {w}x{h}") colors = set() for y in range(h): for x in range(w): if pixels[x,y][3] > 0: colors.add(pixels[x,y]) print("Colors:", colors)